Simultaneous Determination of 8 Ingredients in Guizhijiashaoyao Decoction by HPLC / 中国药房

OBJECTIVE： To establish a method for simultaneous determination of albiflorin， paeoniflorin， liquiritin， liquiritigenin， cinnamic acid， cinnamaldehyde， ammonium glycyrrhetate and 6-ginger phenol in Guizhijiashaoyao decoction. METHODS： HPLC method was adopted. The determination was performed on Kromasil C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelengths were 235 nm（0-35 min，albiflorin， paeoniflorin， liquiritin）、280 nm（35-65 min，liquiritigenin， cinnamic acid， cinnamaldehyde， ammonium glycyrrhetate and 6-ginger phenol）. The column temperature was 25 ℃， and the sample size was 20 μL. RESULTS： The linear range of albiflorin， paeoniflorin， liquiritin， liquiritigenin， cinnamic acid， cinnamaldehyde， ammonium glycyrrhetate and 6-ginger phenol were 2.125-34.000 μg/mL（r＝0.999 9）， 28.700-459.200 μg/mL（r＝0.999 7）， 3.675-58.800 μg/mL（r＝0.999 7）， 1.235-19.760 μg/mL（r＝0.999 8）， 2.300-36.800 μg/mL（r＝0.999 8）， 0.955-15.280 μg/mL（r＝0.999 7）， 36.000-576.000 μg/mL（r＝0.999 7） and 1.500-24.000 μg/mL（r＝0.999 7）， respectively. The quantitative limits were 0.135， 0.102， 0.096， 0.033， 0.013， 0.023， 0.663， 0.198 μg/mL； the detection limits were 0.041， 0.031， 0.029， 0.010， 0.004， 0.007， 0.201， 0.059 μg/mL. RSDs of precision， stability and reproducibility tests were all lower than 3％ （n＝6）. The recovery rates were 97.47％-100.76％（RSD＝1.33％，n＝6）， 98.15％-103.50％（RSD＝1.82％，n＝6）， 95.65％-100.84％（RSD＝2.38％，n＝6）， 96.75％-100.32％（RSD＝1.31％，n＝6）， 95.88％-102.75％（RSD＝2.52％，n＝6）， 95.63％-100.63％（RSD＝2.00％，n＝6）， 96.78％-100.45％（RSD＝1.35％，n＝6）， 95.71％-100.48％（RSD＝1.80％，n＝6）. CONCLUSIONS： The method is accurate， reliable and exclusive， and suitable for simultaneous determination of 8 ingredients in Guizhijiashaoyao decoction.

Effects of Membranous Milkvetch (Astragalus membranaceus)on the Action of "Decoction of Invigorating Yang for Recuperation / 中草药

A stragalus membranaceus(AM ) plays an important role in the traditional Chinese recipe" Decoction of Invigorating Yang for Recuperation" (DIC ) as the main ingredient which invigorates Qi and promotes blood circulation. But in clinics,doses of AM used in tbe recipe of ten varies. For the purpose to investigate the effect of different doses of AM on the action of DIC, animal experiments were designed and studied. Rsults showed that when the doses of AM were 15 and 30g,the resulting DIC showed a better antiirritability,and enhancing immunity effects,as well as in vivo antithrombosis and vitro anticoagulation actions as compared to a dose of 120g AM in DIC (P

Study on quality standard for Shexiangtongbi Babu Plaster / 中成药

AIM: To establish the quality standard for Shexiangtongbi Babu plaster (Radix Astragali, Radix Angelicae sinensis, moschus, Commiphora myrrha, Resina Boswelliae Carterii, Radix Angelicae Pubescentis, Sanguis Draxonis, etc.) METHODS: Borneolum Syntheticum, Commiphora Myrrha, Radix Angelicae Pubescentis, Sanguis Draxonis, Radix Angelicae Sinensis were identified by TLC, and the content of Muscone was determined by GC. RESULTS: Borneolum Syntheticum, Commiphora Myrrha, Radix Angelicae Pubscentis, Radix Angelicae Sinensis could be identified by TLC. Muscone showed a good linear relationship at range of 0.026?g～0.078?g, r =0.9992. The average recovery was 97.57%, and RSD 1.50% respectively. CONCLUSION: The method is accurate and can be used to control the quality of Shexiangtongbi Babu plaster.